Abstract:
Exopolysaccharides (EPS) are secondary metabolites produced by
microorganisms, EPS is produced by lactic acid bacteria (LAB) under
unfavorable conditions. The purposes of this study were to select LAB capable
of producing EPS and to determine the optimal time, temperature, and medium
required by LAB to produce EPS. This study used 5 isolates, JN2, JN5, JN6,
S3PP8, and S3PP12. In the selection stage of LAB producing EPS in 2% skim
milk liquid media, the five isolates did not produce EPS. Selection results using
MRSA added with sucrose showed that 1 isolate (JN6) produced mucoid EPS,
while 4 isolates (JN2, JN5, S3PP8 and S3PP12) produced mucoid and ropy
type EPS. EPS production test of S3PP12 isolates on MRSB-glucose and
MRSB-sucrose medium showed that LAB could not produce EPS.
