SINTESIS DAN UJI TOKSISITAS SENYAWA PIRAZOLIN 5-(4-BROMOFENIL)-3-(1- HIDROKSINAFTALEN-2-IL)-1-FENIL-4,5-DIHIDRO-1H-PIRAZOL

Abstract

Pyrazoline is a secondary metabolic compound with five circular heterocyclic structures that has three carbon atoms and two nitrogen atoms position close to each other. Pyrazoline has high toxicity and possess various bioactivities such as antitumor and anticancer agent. Pyrazoline (5- (4-bromophenyl)-3-(1-hydroxynaphthalene-2-yl)-1-phenyl-4,5-dihydro-1H-pyrazole compound has successfully synthesized with two phases of reaction. The reaction begins formation of chalcone 3-(4-bromophenyl)-1-(1-hydroxynaphthalene-2-yl)-2-propen-1-on by reacting 1- hydroxy-2-acetylnaphthalene compound with 4-bromobenzaldehide. Chalcone synthesis through claisen-schmidt condensation reactions with yield 85,86%. The next step was reacted chalcone with phenilhydrazine to produce target compound used sodium hydoxide catalyst. The target compound synthesis method was sealed-vessel reactor used monowave then produce yield 41,07%. Pyrazoline compound ware purified by recrystallization then tested for purity with TLC test, melting point and HPLC analysis and confirming its structure based on spectroscopic data of UV and FTIR. The synthesized product was screened for toxicity capability by brine shrimp lethality test (BSLT) using Artemia salina Leach larvae. Pyrazoline compounds have LC50 value for pyrazoline are 8,38 μg/mL which can be interpreted as the synthesized compounds are very toxic.