Abstract:
The effect of consuming fructose sugar on health in excessive amounts has been controversial in
recent years, this is because the use of fructose sugar in food and beverages has a negative impact
on health such as increasing the risk of obesity and diabetes mellitus. This study used a biosensor
with amperometric principles to determine the effect of different types of sugar on the yeast
Saccharomyces cerevisiae as a cell model. Measurements were made by adding controlled sugar to
the metabolic activity of cells using an amperometric biosensor based on Pt/Ag electrodes.
Amperometric biosensor will measure the current from a reduction and oxidation reaction with a
constant potential. The effect of added sugar on yeast cells was observed in the form of cellular
respiration which was expressed as a parameter of dissolved oxygen level (DO). The sugar used as
a sample is fructose sugar with variations in concentrations of 0.1 M, 0.2 M and 0.3 M. The
addition of fructose sugar causes an increase in the potential value of 2.34% which indicates DO
levels in the cell environment have decreased due to yeast cells consuming O2 during the respiration
process. The results of this study indicate that the biosensor can detect variations in the
concentration of Saccharomyces cerevisiae yeast cells.
