ANALISIS FILOGENETIK NIBUNG (Oncosperma tigillarium (Jack) Ridl.) “FLORA MASKOT” RIAU BERDASARKAN SEKUEN GEN rbcL DENGAN METODE MAXIMUM PARSIMONY (MP)

Abstract

Taxonomic studies using morphological markers on nibung are difficult to do, because generative characters such as flowers and fruits as important characters are difficult to find in the out of season period. Thus, an alternative analysis using molecular methods is required. The study aimed to analyze kinship relationships and to reconstruct phylogenetic trees based on the character of the rbcL gene. DNA Extraction Procedure used CTAB method with modification. DNA fragments were sequenced at the First Base Laboratories Malaysia through PT. Genetika Science Indonesia followed by alignment using Bioedit application and phylogenetic analysis using Maximum Parsimony (MP) methods in PAUP* application version 4.0a169. The MP analysis grouped three collections of Oncosperma tigillarium into monophyletic clads with a bootstrap value of 100%. This grouping shows that the three collections of Oncosperma tigillarium from Bengkalis Island and Oncosperma tigillarium from Rupat Island have a close kinship based on rbcL genes