ANALISIS FILOGENETIK TIGA KULTIVAR SALAK DI DAERAH ACEH BERDASARKAN PENANDA rbcL MENGGUNAKAN METODE NEIGHBOR JOINING

Abstract

Aceh is one of the center areas of wild type and cultivated snake fruit (salak) as it has a various topography. The massive deforestation resulted in reduced salak habitat and salak diversity in Aceh. In addition, lack of information about salak kinship in Aceh and difficulty in morphological-based taxonomic studies lead to the requirement of molecular approach which facilitate the study of salak taxonomy. This study aimed to reconstruct phylogenetic trees and analyze the kinship relationship of Salacca species in Aceh based on rbcL gene sequence using neighbor joining method. DNA extraction used CTAB method with modification. Phylogenetic analysis using neighbor joining method showed that Salacca sp. (NH.07) Lembah Seulawah had the longest evolutionary rate compared to other two salak samples.