Abstract:
Rhizophora mucronata is a type of mangrove that has a variety of secondary metabolite
compounds. Secondary metabolites in Rhizophora mucronata include alkaloids,
flavanoids, tannins, and other phenol compounds. This research have been isolated
secondary metabolites of Rhizophora mucronata mangrove leaves as well as toxicity
tests by BSLT method. Dried mangrove leaves are then extracted using maceration
method with ethanol solvent for 24 hours and filtered, the maceration process is
repeated 5 times. Ethanol crude extract as much as 97.36 g is separated by gravitational
column chromatography and obtained in the form of dirty crystals on vials 50-70, to get
pure crystals done recrystallized and obtained compounds coded Rm-FE yellow crystals
with melting point 172-174 ºC. Phytochemical tests conducted on Rm-FE compounds
showed a discoloration from yellow solution to pink solution, discoloration showed
positive compounds of flavonoid group. Rm-FE UV spectrum indicates the presence of
absorption at λmax 274 nm, while the FT-IR spectrum shows peaks at waves of
1053-1141 cm-1 (C-O), 1606-1635 cm-1 (C=C), 2912–2936 cm-1 (C-H aliphatic), and
3309 cm-1 (O-H). Toxicity test results with BSLT (Brine Shrimps Lethality Test)
method against ethanol coarse extract from Rhizophora mucronata leaves are non-toxic.
