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ANALISIS TIGA METODE PENENTUAN AKTIVITAS ENZIM α-AMILASE

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dc.contributor.author Az Zahra, Elmerrillia Sylvia Haning
dc.date.accessioned 2021-10-19T02:20:00Z
dc.date.available 2021-10-19T02:20:00Z
dc.date.issued 2021-03
dc.identifier.other wahyu sari yeni
dc.identifier.uri https://repository.unri.ac.id/handle/123456789/10263
dc.description.abstract The enzyme activity as a biocatalyst can be calculated based on the number of moles of the substrate that is hydrolyzed into product per unit time (Iodine method), or the number of moles of product produced per unit time (2,3-dinitrosalicylic acid (DNS) method and the Nelson-Somogyi (NS) method). This third method are with experimental conditions of pH 7, temperature of 50°C and an incubation time of 30 minutes. The results showed that the α-amylase enzyme activity measured by the DNS method was 0.115 ± 0.002 μmol/minute and the NS method was 0.041 ± 0.025 μmol/minute, while the Iodine method had activity of 2.090 ± 0.005 μmol/minute. The activity data of these three methods after being statistically tested by the Duncan method turned out to be significant between the DNS method (2,3-dinitrosalicylic acid) with the Nelson-Somogyi (NS) method and the Iodine method. en_US
dc.description.provenance Submitted by wahyu sari yeni (ayoe32@ymail.com) on 2021-10-19T02:20:00Z No. of bitstreams: 1 Elmerrillia Sylvia H._compressed.pdf: 192711 bytes, checksum: 9c71b701ea85534935a3e886d9258fb2 (MD5) en
dc.description.provenance Made available in DSpace on 2021-10-19T02:20:00Z (GMT). No. of bitstreams: 1 Elmerrillia Sylvia H._compressed.pdf: 192711 bytes, checksum: 9c71b701ea85534935a3e886d9258fb2 (MD5) Previous issue date: 2021-03 en
dc.description.sponsorship Bidang Biokimia Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau en_US
dc.language.iso en en_US
dc.subject 3,5-dinitrosalicylic acid en_US
dc.subject α-amylase enzyme en_US
dc.subject iodine en_US
dc.subject Nelson-Somogyi en_US
dc.subject strach en_US
dc.title ANALISIS TIGA METODE PENENTUAN AKTIVITAS ENZIM α-AMILASE en_US
dc.type Article en_US
dc.contributor.supervisor Devi, Silvera


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