ISOLASI METABOLIT SEKUNDER DAN UJI ANTIBAKTERI EKSTRAK n-HEKSANA DARI DAUN BAKAU Rhizophora apiculata (Rhizophoraceae)

Abstract

Mangrove have several compounds that can be used as antibacterial, one of them is Rhizophora apiculata. The purpose of this study was to isolate secondary metabolites and determine the antibacterial activity from R. apiculata leaves extract. This study used a maceration method with ethanol solvent which was then extracted using hexane. Separation of the extract was carried out by VLC. Fraction F4 resulted in a compound that coded RA-02 in a white solids and decomposed at 108-109 ºC. UV spectrum of RA- 02 indicated absorption at λmax 203 and 312 nm. The FT-IR spectra indicate the peak at wave number (cm-1) are 1165-1246 (C-O-C) ether, 1490 and 1458 (C-H methylen and methyl), 1598 (C=C) and 2852 –2935 (C-H) aliphatic. Antibacterial activity test using agar diffusion method showed that hexane extract can inhibited the growth of Escherichia coli ranges from 11,4 ± 4,22 to 13,77 ± 4,25. These results showed that mangrove plants have the potential to be developed as an antibacterial agent