ISOLASI BAKTERI PENGHASIL SELULOSA DARI LIMBAH INDUSTRI KARET ASAL RIAU DAN BUAH-BUAHAN TROPIS (MANGGIS DAN KAPULASAN): APLIKASI UNTUK PRODUKSI KERTAS

Abstract

The use of cellulose from plants for paper production continuously requires energy and can damage the environment, so an alternative source of renewable cellulose is needed with more efficient results, namely the production of cellulose from bacteria. Therefore, this study aimed to isolate and test cellulose-producing bacteria from mangosteen, durian, kapulasan and industrial rubber waste for paper production applications. The method used was the isolation of cellulose bacteria using the Hestrin Schramm (HS) medium, the selection of selected isolates using the Glucose Extract Yeast (GEY) medium, then A production test was carried out using a modified coconut water medium under static conditions for 14 days and the determination of bacterial cellulose production. The results showed that as many as 6 isolates (MSB-1b, MSB-2b, MSB-5b, PULR-1b, PULR-2b and PULR-2c) that were tested were successful in forming cellulose mats and none were successful in forming cellulose pellicles. The z / k ratio values of each isolate were MSB-1b = 2.15, MSB-2b = 2.70, MSB-5b = 2.85, PULR-1b = 5.93, PULR-2b = 5.30 , and PULR-2c = 5.10. Based on the research results, the z / k ratio cannot be used as a reference in selecting the ability of cellulose bacteria to form pellicles, so it cannot be known about its ability as a material for paper production.