AKTIVITAS ANTIBAKTERI METABOLIT SEKUNDER AKTINOMISETES YANG DIEKSTRAKSI MENGGUNAKAN ETIL ASETAT

Abstract

Infection from pathogenic bacteria can cause various diseases in humans, such as diarrhea. The diarrheal disease can be caused by bacteria Escherichia coli, Salmonella typhimurium, and Shigella flexineri. Meanwhile, actinomycetes are reported to be one of the producers of antibiotics. The actinomycetes used were isolated from soil in the Universitas Riau Arboretum, namely isolates B1.06, B3.13, C2.18, A2.01, and C2.20. The purpose of this study was to study the antibacterial activity of secondary metabolites of Actinomycetes extracted using ethyl acetate against pathogenic bacteria that cause diarrheal diseases. The disk diffusion method was used for testing antibacterial activity. From the results, it was found that the actinomycetes isolate was able to overcome the pathogenic bacteria, with a diameter of inhibition zone formed was 8.2 mm against Salmonella typhimurium and 20.6 mm against Shigella flexineri. The diameter of the inhibition zone formed was compared with the tetracylcline and produced antibacterial activity criteria of actinomycetes isolates that were resistant, intermediate and sensitive.