Abstract:
Flacourtia rukam is one of the species of Flacourtiaceae which is widely spread in
Indonesia. This plant commonly used as traditional medicine by various tribes in
Indonesia. The study is to isolate secondary metabolite compound from combine fraction
with Vacuum Liquid Chromatography (VLC) method and Flash Chromatography
method from sub fraction F3. The purity of the compound was analyzed by TLC and
melting point. The melting point was obtained at 120-122oC. The isolated compound
from extract n-hexane rukam leaf was characterized by FT-IR spectroscopy, which
showed the presence of the OH group, CH2 (methylene), CH3 (mehyl), aliphatic C-H
and C-O (alcohol). FR-H-02 revealed as a steroid β-Sitosterol after the spectroscopy data
were compared with published data. Combine fraction was toxic, based on the toxicity
test showed the LC50 values of 776,24 ppm. Toxicity test of β-Sitosterol showed that
LC50 value >1,000 ppm which indicated the compound was not toxic against Artemia
salina .