DSpace Repository
OPTIMASI SUHU ANNEALING PASANGAN PRIMER UNTUK AMPLIFIKASI DAERAH ETS (EXTERNAL TRANSCRIBED SPACER) PADA TUMBUHAN DURIK-DURIK (Syzygium sp.) ASAL RIAU
- Home
- →
- UR - Student Paper Degree
- →
- Faculty of Mathematics and Natural Sciences
- →
- Biology
- →
- View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.
| dc.contributor.author | Aisyah, Nur | |
| dc.date.accessioned | 2021-07-08T03:23:19Z | |
| dc.date.available | 2021-07-08T03:23:19Z | |
| dc.date.issued | 2020-06 | |
| dc.identifier.other | wahyu sari yeni | |
| dc.identifier.uri | https://repository.unri.ac.id/handle/123456789/10003 | |
| dc.description.abstract | PCR (Polymerase chain reaction) is a technique for amplification target genes using specific primers. The PCR process consists of three stages, namely denaturation of template DNA, annealing and polymerization of DNA chain (extensions). The successful of a PCR is influenced by the PCR components and primer annealing temperature. This research was conducted to obtain optimal annealing temperature for the amplification of the ETS region. The PCR components were such as DreamTaq DNA Polymerase (Thermo Scientific) and a primer of Myrtf and 18SR. The optimal annealing temperature for PCR of the ETS region was 56,8°C. The amplicon obtained in this study was approximately 500 bp | en_US |
| dc.description.provenance | Submitted by wahyu sari yeni (ayoe32@ymail.com) on 2021-07-08T03:23:19Z No. of bitstreams: 1 REPOSITORY NUR AISYAH_compressed.pdf: 152063 bytes, checksum: f14a03a1b85c307ece839fce4091b44a (MD5) | en |
| dc.description.provenance | Made available in DSpace on 2021-07-08T03:23:19Z (GMT). No. of bitstreams: 1 REPOSITORY NUR AISYAH_compressed.pdf: 152063 bytes, checksum: f14a03a1b85c307ece839fce4091b44a (MD5) Previous issue date: 2020-06 | en |
| dc.description.sponsorship | Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Riau | en_US |
| dc.language.iso | en | en_US |
| dc.subject | annealing temperature | en_US |
| dc.subject | DNA polymerase | en_US |
| dc.subject | ETS region | en_US |
| dc.subject | PCR | en_US |
| dc.subject | Syzygium | en_US |
| dc.title | OPTIMASI SUHU ANNEALING PASANGAN PRIMER UNTUK AMPLIFIKASI DAERAH ETS (EXTERNAL TRANSCRIBED SPACER) PADA TUMBUHAN DURIK-DURIK (Syzygium sp.) ASAL RIAU | en_US |
| dc.type | Article | en_US |
| dc.contributor.supervisor | Roslim, Dewi Indriyani |
Files in this item
This item appears in the following Collection(s)
-
Biology [401]