Nuraini, Intan Sari2021-07-082021-07-082020-06wahyu sari yenihttps://repository.unri.ac.id/handle/123456789/9999Annealing temperature is a temperature that is required for primer annealing in DNA template. This temperature is very crucial for the PCR success. This study aims to determine the appropriate annealing temperature for amplifying of atpB-rbcL intergenic spacer on Benstonea sp. The PCR components use Dream Taq DNA Polymerase (Thermo Scientific) and a primer pair of atpB_F and rbcL_R. The optimal annealing temperature for PCR of the atpB-rbcL intergenic spacer was 50,2 °C. The amplificon on this annealing temperature was single, thick and the size is right.enannealing temperatureatpB-rbcL intergenic spacerBenstonea spPCRArticle