Siregar, Elsa Novita Br2023-08-102023-08-102023-06PerpustakaanElfitrahttps://repository.unri.ac.id/handle/123456789/11112One of the most commonly found organic waste is plastic bags. Plastic is an inorganic waste that has constituent components that are difficult to decompose in nature. The use of microorganisms such as bacteria and the use of enzymes is an effective and environmentally friendly way. The purpose of this study was to determine the ability of Riau local isolate bacteria in degrading black High Density Polyethylene (HDPE) plastic bags. Rejuvenation of bacterial isolates is carried out to ensure the isolates used are appropriate. The biodegradation test was based on the percentage weight reduction of HDPE plastic sheets on the medium for 60 days. The results showed that the isolates had HDPE biodegradation activity that was able to degrade black plastic within 60 days incubation time. The percentage of weight reduction of the test plastic was by LSSB 2.3 isolate of (0.35%).enBiodegradationplasticbacteriaHigh Density Polyethylene (HDPE)Riau local isolateArticle