Juliani, R.YuharmenTeruna, H. Y2014-03-272014-03-272014-03-27Rangga Dwijunanda Putrahttp://repository.unri.ac.id/xmlui/handle/123456789/5930Soursoup (Annona muricata L) was used in herbal medicine. This Annonaceae family member contains alkaloids, tannins, and several other chemical constituents, including asetogenin that allegedly to have cytotoxic potential. The n-hexane and methanol macerating method was applied to isolate the secondary metabolites from the stem bark of this plant. The separation was carried out by vacuum liquid chromatography (VLC), column chromatography, and gel chromatography respectively. Characterization of the fractions was established using UV-Vis and FTIR spectrophotometry. Toxicity assay was conducted by Brine Shrimp Lethality Test (BSLT) method. The LC50 of 3rd to 11th fractionts of the methanol extract wer 4481.25; 37; 11.46; 0.85; 3.02; 0.23; 10.97; 8.53 and 4093 ppm, respectively. The 3rd and 11th fractionts were not toxic, whereas other fractions were toxic and potential to be used as an anticancer.otherAnnona muricata LBSLTFTIRtoxicityOther