Dewi, Ratna Reski2022-03-152022-03-152021-10wahyu sari yenihttps://repository.unri.ac.id/handle/123456789/10490Hydrazone is a versatile compound in drug design for the synthesis of heterocyclic compounds with different biological activities because it has two active centers, namely two nitrogen atoms and two carbon atoms. The combination of hydrazones with other functional groups produces compounds with unique physical and chemical properties. The addition of a fluoro group in a compound can have a significant effect and can increase the activity of the compound. Some of the pharmacological activities of hydrazone compounds include antimicrobial, antiviral, anticancer and others. The aim of this study was to synthesize the compound N'1,N'3-bis((E, Z)-4-fluorobenzyliden) malonohydrazide which has a good toxicity value. The compound N'1,N'3-bis((E,Z)-4-fluorobenzyliden) malonohydrazide was synthesized by condensation reaction between malonohydrazide and 4-fluoro benzaldehyde using glacial acetic acid as catalyst. Synthesis of compound N'1,N'3- bis((E, Z)-4-fluorobenzyliden) malonohydrazide using reflux with heating and stirring method. The purity test for this compound was determined using the TLC test, melting point determination and HPLC analysis. The structure of the compound was identified using spectroscopic analysis of UV and FTIR. The yield obtained from the synthesis was 67.79%. The toxicity test of the compound was carried out using the Brine Shrimp Lethality 2 Test (BSLT) method and the results showed that this compound was toxic with an LC value of50 = 125.02 g/mL.enBSLTfluorohydrazoneArticle