Kurniawan, Irfan Ari2023-01-262023-01-262022-10PerpustakaanElfitrahttps://repository.unri.ac.id/handle/123456789/10826White lotus (Nymphaea nouchali Burm f) is one of the natural ingredients used as traditional medicine. Therefore, it is necessary to conduct a scientific study of the chemical content and its effects. This study aimed to isolate secondary metabolites and to test the toxicity of lotus tuber (N. nouchali) from nusing the Brine Shrimp Lethality Test (BSLT) method. The phytochemical test results contained terpenoid, saponin and phenolic secondary metabolites. The isolation used was maceration with n-hexane solvent as much as 1.5 kg of crude sample obtained 9.55 g of thick sample. The obtained macerates were separated by Vacuum Liquid Chromatography (VLC) method, so that 4 different fractions were obtained based on the Rf of TLC. In Fraction 3 (F3) code NNBFH- F3 white needle-shaped crystals were obtained and recrystallized. The crystal has a melting point of 128-130 oC. FT-IR spectra were obtained at wavelengths of 3268 cm-1 (- OH), 1654 cm-1 (C=C), 2937 cm-1 (aliphatic C-H), 1464 and 1382 cm-1 (CH2 and CH3), 1062 cm-1, and 1054 cm-1 (CO). Based on the results of the toxicity test using the BSLT method, the LC50 value is >1000 ppm showed that the compound obtained was non-toxic.enhexanelotustoxicityArticle