Nurhayati, Siti2021-07-082021-07-082020-06wahyu sari yenihttps://repository.unri.ac.id/handle/123456789/10005Pandan (Benstonea sp.) is one of the most important plant in Kajuik Lake located in Langgam, Riau Province, Indonesia. Polymerase Chain Reaction (PCR) is a technique used to multiply or amplify DNA samples in vitro using a primer pair. This study aims to optimize annealing temperature of trnQ-5’rps16 intergenic spacer primer. Stages of research are total DNA isolation using Genomic DNA Mini Kit Plant (Geneaid), PCR and electrophoresis. The result showed that the optimal annealing temperature for amplification of trnQ-5’rps16 intergenic spacer is 58°C. The PCR product on this annealing temperature is thick.enAnnealing temperatureBenstonea spPCRRiautrnQ-5’rps16 intergenic spacerArticle