Sianipar, Evlin Magdalena2022-12-152022-12-152022-08PerpustakaanElfitrahttps://repository.unri.ac.id/handle/123456789/10788Biodegradation is one of the strategies for the handling of PET bottle waste by involving the activity of microorganisms, such as bacteria. In this study, the thermophilic bacteria LBKURCC191, 192, 193, 194, 195 and 196, which have been isolated from hot springs of Bukik Kili, Solok-Sumatera Barat, acts as degrading agents for PET bottles. The six bacteria isolates were tested for their ability to degrade mineral water bottles “Le Minerale” using the solid fermentation method for four weeks. LBKURCC191 (Bacillus sp) was isolate with the highest percentage of PET weight loss. These bacteria were able to degrade PET up to 0,620% on NA media and 0,443% on MSM media. SEM analysis on PET incubated with LBKURCC191 bacteria showed changes of surface structure to become rough, bumpy and uneven due to enzyme activity by bacteria during incubation. FTIR analysis showed shifting of wavenumber to a lower value in the absorption of ester, alcohol and C-H aliphatic, so the bond length increased and bond strength decreased. This fact shows the stretching of the bonds on several functional groups in the PET structure due to the activity enzymes produced by bacteria for degradation.enBiodegradationenzymePETArticle