Haura, Sari Rakha Al2022-12-262022-12-262022-08PerpustakaanElfitrahttps://repository.unri.ac.id/handle/123456789/10805Lipolytic bacteria are bacteria that produce lipase enzymes to hydrolyze fats into fatty acids and glycerol. Lipolytic bacteria can be found in oil-contaminated habitats such as palm oil mill effluent. One of the applications of lipase enzymes is as biodetergent formulations that are eco-friendly. This research aimed to obtain potential lipolytic bacteria isolates from palm oil mill effluent and analyze the potential of their lipase to be applied as a biodetergent formulation through several tests, namely enzyme activity test, enzyme activity stability test and washing test. The results obtained 22 isolates of lipolytic bacteria with one isolate of potential lipolytic bacteria. LSSB 4.1 isolate produced lipase activity of 4.125 U/ml, produced a relative activity of 88% when the isolate was exposed to 1% detergent and could remove oil from cotton fabric with a percentage of 61.3%. Lipase from LSSB 4.1 isolates has the potential to be developed as a biodetergents formulation.enBiodetergentlipase activitylipolytic bacteriapalm oil mill effluentwashing testArticle