RusliNugroho, Titania TjandrawatiSaryono2016-05-102016-05-102016-05-10wahyu sari yenihttp://repository.unri.ac.id/xmlui/handle/123456789/8359β-Galactosidase is an enzyme that can catalyze the lactose hydrolysis reaction into glucose and galactose. This study was carried out to determine the ability of endophyte fungi Fusarium oxysporum LBKURCC41 to produce β-galactosidase enzyme. This fungi was grown in production media : lactose 10 g, yeast extract 20 g, MgSO4 1 g and K2HPO4 1 g per liter destilat water. The β-galactosidase activity of the enzyme was determined based on the amount of oNP (o-nitrophenol) liberated in Unit/mL from the substrat oNPG (o-nitrofenil-β-D-galaktopiranosida), while the specific activity of the enzyme was indicated by activity per unit weight of proteins. The protein concentration of enzyme extracts was determined by the Lowry method. The results showed that the fungi tasted was able to produce enzyme β-galactosidase. Enzyme activity of isolate LBKURCC41 at 2, 4, 6, 8, and 10 days of production time were 0.146 ± 0.060 Unit/mL, 0.150 ± 0.058 Unit/mL, 0.134 ± 0.040 Unit/mL, 0.137 ± 0.037, and 0.132 ± 0.075 Unit/mL respectively. No real difference activity for each of production time base on ANOVA α≥0,05. Specific activity of the isolate was 1.568 ± 0.140 Unit/mg protein.enβ-Galactosidaseendophyte fungiFusarium oxysporumstudent Paper Post Degree