Ariwayeni, Winda2015-07-082015-07-082015-07-08Dodyhttp://repository.unri.ac.id/xmlui/handle/123456789/7311Pineapple waste ((leather: Pineapple core: Waste pineapple pulp) can to be processed into acetic acid using Kombucha mushroom starter. The research aimed to analyzed the optimization of the use of the substrate, the starter and fermentation time. Fermentation is use through variation of substrat and starter, so that obtained the optimum of the use of the substrate is 50%, 45 grams of starter which are fermentated with optimum time for 5 days, acetic acid content is determined by using a High Performances Chromatografi Liquid (HPLC) with resulting 4.23%.en-USAcetic acidPineapple wasteKombucha.UR e-Research