SINTESIS DAN UJI TOKSISITAS (E)-3-(4-((E)-((4-HIDROKSIFENIL) IMINO)METIL)FENIL)-1-(2-METOKSIFENIL)PROP-2-EN-1-ON

Abstract

Chalcone is a compound that has two aromatic rings in its structure and connected by 3 carbon atoms which is an unsaturated α, β carbonyl system. Chalcone conjugated is the conjugation of two bioactive molecules as one of the strategies to obtain new bioactive compounds. Chalcone conjugate (E)-3-(4-((E)-((4-hydroxyphenyl)imino)methyl) phenyl)-1-(2-methoxyphenyl)prop-2-en-1-one was synthesized from (E)-4-(3-(2-methoxy phenyl)-3-oxoprop-1-en-1-yl)benzaldehyde and 4-aminophenol by microwave irradiation method. The purity of the chalcone conjugate was determined by the TLC test, determination of melting point, and HPLC analysis. The structure of the chalcone conjugate was obtained from UV, FTIR, NMR, and HRMS spectroscopic studies. Synthesis of chalcone conjugate in this research was concluded with a yield 33,60%, and the toxicity was determined by the Brine Shrimp Lethality Test (BSLT) method. The value of the LC50 was showed 6,525 μg/mL. From the data above was concluded that chalcone conjugate was toxic.