SINTESIS DAN UJI TOKSISITAS ANALOG PIRAZOLIN (E)-7-(4-METOKSIBENZILIDEN)-3-(4-METOKSIFENIL)-2-FENIL-3, 3a, 4, 5, 6, 7-HEKSAHIDRO-2H-PIRAZOLO [4,3c] PIRIDIN

Abstract

Pyrazoline is five ring heterocyclic compounds with two adjacent nitrogen atoms which is known to have diverse biological activities such as antibacterial, antimalarial, antituberkular, anticancer and antioxidant. This study aims to synthesize derivatives of pyrazoline compounds to add variety and increase their bioactivity. Pyrazoline analogue (E)-7-(4 methoxybenzyliden)-3-(4-methoxyphenyl)-2-phenyl-3, 3a, 4, 5, 6, 7-hexahydro-2H-pyrazolo [4,3-c] piridine (FH-NHP-Pi) was synthesized of curcumin 5-bis ((E)-4-methoxybenzyliden) piperidine -4-on and phenylhydrazine using sodium hydroxide as catalyst. Curcumin was prepared by condensing 4-piperidone monohydrate hydrochloride and 4-methoxybenzaldehide using NaOH as catalyst. Pyrozoline FH-NHP-Pi were synthesized via sealed vessel reactor with stirring speed 1000 rpm 80oC yield of 89,29 %. Purity of the compounds were analyzed using TLC, melting point, and HPLC. The structure of synthesized compounds were confirmed by UV, FTIR, NMR and HRMS spectrocopy studies. The toxicity of pyrazoline were evaluated by Brine Shrimp Lethality Test (BSLT) method. The result of the test showed pyrazoline FH-NHP-Pi is considered non-toxic were 217,3 µg/mL.