Isolation Cellulolytic Bacteria Degradation Of Empty Fruit Bunch Of Oil Palm In Peatland

Abstract

The using of a peatland as the food crops cultivationhave been motivate the part of an agriculture to more developing the agricultural innovations, such as the making of an organic fertilizer that can be used by the food crops in the peatland. An organic material that can be used as the raw material to make an organic fertilizer, which one it is the empty fruit bunch of oil palm (EFBP). It is something like the compost heap or the waste of oil palm trees, which is contain of cellulose with the result that the substrate for the growth of the cellulolytic bacteria. The election of EFBP as the source of the isolate is because there is many oil palm trees which is cultivated on the peat land and it is naturally degraded around the palm trees. The natural degradation of EFBP will require along time enough. Therefore, this study is purposed to isolating the cellulolytic bacteria from EFBP and to obtain bacteria that having a high cellulolytic index and also acid-resistant bacteria, and later could be used to remodel the EFBP itself as well as the other organic mater inthe peatland. Based on the isolation result and morphology observation according to gram coloring, it is obtained 12 isolates which there is 4 isolates that negative Gram cocci form, 1 isolate that positive Gram cocco form, 2negative Gram basil, 2 gram negative diplobasil, 2 positive Gram basil, 1 negative Gram basil. Furthermore to do the measuring of celulolytic is to know the ability of the bacteria of producing an cellulase enzyme that pass through the clear zone which is formed around the colony. Based on the observation, the highest amount of cellulolytic index is 7 which are obtained form the diplobasil shaped positive gram. The best time to apply the making of organic fertilizer which one is should be done on the third day of incubation