ISOLASI DAN UJI AKTIVITAS ANTIMIKROBA SENYAWA METABOLIT SEKUNDER DARI EKSTRAK KULIT MELON (Cucumis melo L)

Abstract

This study aims to determine the presence of secondary metabolites contained in the melon peel and their antimicrobial activity. Sample was extracted by maceration method using hot aquadest (60℃). The gravity column chromatography of the melon peel extract gave 5 combined fractions. These fractions were subjected to antimicrobial test and FTIR was recorded. Antimicrobial activity test using agar diffusion method with a concentration of 600, 800 and 1000 μg/disc for the extract and 1000 μg/disc for the fractions. Identification showed the presence of terpenoid and phenolic compounds. From 1 kg of melon peel gave 61.85 g of black gummy extract and produced 5 yellow liquid fractions. Melon peel extract showed weak activity againts B. subtilis and P. aeruginosa, but not active against A. niger.