Abstract:
The study aims to biosynthesis of silver nanoparticles using extract of Zingiber officinale rhizome. This methode was performed by reducing AgNO3 at room temperature in 45 hours with secondary metabolites of extract rhizomes. The formation of silver nanoparticles was confirmed by doing characterization using UV-Vis spectrophotometer. The silver nanoparticles can be observed at 402, 404, 412, 411, 417 and 419 nm from the UV–vis spectrum for ratio 1:1; 1:3; 1:4; 1:5; 1:10 and 1:20 (exract: AgNO3). Antibacterial activity was performed against Staphylococcus epidermidis and Pseudomonas aeruginosa. The results showed that silver nanoparticles was actively inhibit the growth of Staphylococcus epidermidis. Silver nanoparticles solutions was more active in inhibiting the growth of bacteria tested than aqueous extract of Zingiber officinale and Alpinia galanga