ISOLASI MINYAK ATSIRI DAUN KEMANGI (Ocimum sanctum L) CARA KONVENSIONAL DAN MICROWAVE SERTA UJI AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN

Abstract

The aim of this study was to isolate essential oils from Ocimum sanctum L leaves by Clevenger-hydrodistillation and microwave-assisted hydrodistillation. Components of the essential oil were then identified using Gas Chromatography Mass Spectrometry (GC-MS). Antimicrobial activity of these oils was determined by this diffusion method against Escherichia coli, the main component of the essential oils was geranial (24.74%) and neral (28.11%) for Clevenger-hydrodistillation method, while geranial (41.85%) and neral (29.07%) for the method microwave-assisted hydrodistillation. Our finding for antimicrobial test showed that essential oil of microwave-assisted hydrodistillation was more active than those of Clevenger-hydrodistillation. Antioxidant activity using DPPH assay of the essential oil of Clevenger-hydrodistillation was more active (IC50=513.1802 μg/mL) than those of microwave-assisted hydrodistillation (IC50=713.2279 μg/mL).