ISOLASI DAN UJI AKTIVITAS ANTIJAMUR SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL KULIT BATANG Goniothalamus sp. (ANNONACEAE)

Abstract

The aim of this study was to isolate the secondary metabolites of the stem bark of Gonoiothalamus sp. and to determine antifungal activity of methanol extract. The methanol extract was fractionated using column chromatography. One of the fractions was recrystallized and found as was yellowish crystals (F4). Its HPLC chromatogram compared with HPLC chromatogram of pinocembrin. The result showed that he yellowish crystals (F4) was pinocembrin. Antifungal assay was conducted by the agar diffusion method against Candida albicans and Aspergillus niger. The methanol extract and F4 exhibited weak activity against Candida albicans with inhibition zone 9.03 mm for methanol extract and 8.50 mm for F4, while methanol extract and F4 was inactive againts Aspergillus niger.