Abstract:
The performance of a bioremediation of oily sludge process can be determined by measuring the reduction of contaminant
concentrations and by assessing the treatment's ability to lower toxicity. Land treatment of polycyclic aromatic hydrocarbon
(PAH)-contaminated soil from oily sludge. Acute toxicity, as measured by the Daphnia survival assay, testing
bioremediation medium is very necessary for the health of the soil and water from the pollutants. Bioremediation of oily
sludge using bacteria (pre) and post phytoremediation decline in levels of TPH. Although toxic levels decreased, urgent
toxicity tests on organisms to determine of bioremediation are safe for the environment. The aim of this research is to
observe acute toxicity level LC50-48 hours medium of oily sludge composted by bacteria, post phytoremediation and
influence to reproduction of Daphnia carinata King. To get LC50-48 hours value, used static bioassay according to standard
of APHA (1995) counted with Minitab Probit Analysis. For chronic toxicity test medium of oily sludge prae and post
phytoremediation to reproduction of D. carinata King, counted the total of neonates D. carinata King during 21 days. The
result showed that LC50-48 hour’s value of medium of oily sludge prae phytoremediation is amount 1043,69 ppm and post
phytoremediation is amount 4728, 7 ppm. For chronic toxicity test, total of neonates Daphnia carinata King in medium of oily
sludge pre phytoremediation is amount 34 neonates with frequency of brood 4-6 times and the size 0,75 mm. While post
phytoremediation is amount 37 neonates of brood are 5-7 times and the size 0,77 mm
