ANALISA PEREKAMAN DAN REKONSTRUKSI HOLOGRAFI DIGITAL MIKROSKOPIK PADA KACA PREPARAT MENGGUNAKAN METODE IN-LINE

Abstract

This research applied in-line holography method and digital technique to record and reconstruct hologram. This method was used to create the holograms from two microscopic objects. The recording was done using a laser with a wavelength of 650 nm. The samples in microscopic slide used were cross section of hibiscus flower stem and leaves of citrus, CMOS camera was used as image recorder. The laser power was varied using 5 ND filters with it’s power of 3,6 mW, 2,24 mW, 1,2 mW, 0,36mW 0,047 mW and 0,005 mW and also without ND filter. The distance between beam expander and samples was varied and the magnification of objective lens used were also varied at magnification level of 10x, 40x, and 100x. The results of recording were reconstructed using holovision software and were then analyzed using ImageJ to find the grayscale average value. The final results of this research showed that the higher power of laser resulted in holograms with better clarity by the percentage difference between the highest and the lowest grayscale average value of 75 %. The distance between beam expander and samples influenced the intensity on samples, and the closer the lens to the samples, the higher the intensity on samples resulted in holograms with better clarity. Lens with magnification of 100x produced hologram with the best clarity over all the lens by 28 % of the percentage difference of grayscale average value.