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INDUKSI KALUS DARI EKSPLAN DAUN IN VITRO KELADI TIKUS (Typhonium sp.) DENGAN PERLAKUAN 2,4-D DAN KINETIN

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dc.contributor.author Sitinjak, Marlina Agustina
dc.contributor.author Isda, Mayta Novaliza
dc.contributor.author Fatonah, Siti
dc.date.accessioned 2016-01-26T03:53:51Z
dc.date.available 2016-01-26T03:53:51Z
dc.date.issued 2016-01-26
dc.identifier.other wahyu sari yeni
dc.identifier.uri http://repository.unri.ac.id/xmlui/handle/123456789/7785
dc.description.abstract Rodent tuber (Typhonium sp.) belongs to Araceae that has a potential as medicinal plant. The propagation of rodent tuber was conducted using vegetative propagation from bulbs. This procedure takes a long periode to produce a large quantity of plant. Therefore, an in vitro propagation using callus culture is necessary to solve this problem. This research aimed to determine the best concentration of 2,4-D and kinetin to induce callus of in vitro leaf rodent tuber explants. The research was conducted at the Laboratory Terpadu of Biology, Faculty of Math and Natural Sciences UR from August 2014 to January 2015. This research used in vitro leaft explants of rodent tuber with MS medium. The design used is Randomize Group Design with 10 treatment i.e. (A) Without treatment (Control), (B) 0.5 mg / L 2.4-D, (C) 1 mg / L 2.4-D, (D) 1.5 mg / L 2.4-D, (E) 0.5 mg / L 2.4-D + 0.3 mg / L kinetin, (F) 1 mg / L 2.4-D + 0.3 mg / L kinetin, (G) of 1.5 mg / L 2,4-D + 0.3 mg / L kinetin, (H) 0.5 mg / L 2.4-D + 0.5 mg / L kinetin, (I) 1 mg / L 2.4-D + 0.5 mg / L kinetin, (J) 1.5 mg / l 2,4-D + 0.5 mg / L kinetin. The results showed that explants could not produce callus, the explants response just a swelling leaf explants and browning in leaf margin because of an injuries. The treatment that could give up to 100% swelling response was 2.4-D mg / L single (0.5 mg / L and 1 mg / L) and the combination treatment (0.5 mg / L 2.4-D + 0.3 mg /L kinetin, 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1.5 mg / L 2.4-D + 0.5 mg / L kinetin). The treatments that could maintain 66.67% explants growth was the combination 0.5 mg / L 2.4-D + 0.5 mg / L kinetin and 1 mg /L 2.4-D + 0.5 mg / L kinetin en_US
dc.description.provenance Submitted by wahyu sari yeni (ayoe32@ymail.com) on 2016-01-26T03:53:51Z No. of bitstreams: 1 MARLINA AGUSTINA.pdf: 760057 bytes, checksum: 33247c294ba414ac2f3f5ba4df42d3e4 (MD5) en
dc.description.provenance Made available in DSpace on 2016-01-26T03:53:51Z (GMT). No. of bitstreams: 1 MARLINA AGUSTINA.pdf: 760057 bytes, checksum: 33247c294ba414ac2f3f5ba4df42d3e4 (MD5) en
dc.description.sponsorship Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau en_US
dc.language.iso en en_US
dc.subject callus en_US
dc.subject in vitro en_US
dc.subject kinetin en_US
dc.subject rodent tuber en_US
dc.title INDUKSI KALUS DARI EKSPLAN DAUN IN VITRO KELADI TIKUS (Typhonium sp.) DENGAN PERLAKUAN 2,4-D DAN KINETIN en_US
dc.type student Paper Post Degree en_US


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