Abstract:
Hepatitis B virus (HBV) infection can lead to diverse clinical spectrum of liver disease
including fulminant hepatitis, chronic hepatitis, cirrhosis and hepatocellular carcinoma (HCC).
HBV is currently classified into 8 genotypes (A to H) and 4 HbsAg-subtypes which are adw,
ayw, adr, and ayr. Previous studies showed that HBV genotypes and subtypes showed a distinct
geographic and ethnic distribution. Additionally, HBV genotypes may influence the clinical
manifestation of chronic hepatitis B infection, development of HCC and response to antiviral
treatment The aim of the present study was to investigate the differentiation of HBV genotypes
and subtypes distribution among different clinical status of chronic hepatitis B in Pekanbaru. The
design of present study was analytical cross sectional. HBV genotype was examined based on
homology of S gene firom this study with that of GenBank Database. Gene S was generated by
amplification using polymerase chain reaction (PCR) method, followed by DNA sequencing
using dye-labelled terminator method.
A total of 52 of chronic hepatitis B sera was examined in this study, vMch was
obtained fiwm 10 of HBsAg-positive blood donors, 12 of chronic hepatitis B active patients, 10
of liver cirrhosis patients and 20 of HCC patients. The results showed that genotype C (59.6%)
was the major genotype identified, followed by genotype B (40.4%). Based on clinical status,
genotype C was predominantly found in blood donor (70%) dan liver cirrhosis patients (70%).
While in chronic hepatitis B active and HCC, genotype B and C were comparable. Adr (59.6%)
was the major HBsAg-subtype found in all subjects, followed by adw subtype (38.5%) dan ayw
subtype (1.9%). Based on statistical analysis, there was no significant difference in the
distribution of HBV genotypes and subtypes among blood donor, chronic hepatitis B active
patients, liver ciniiosis patiraits and HCC. HOWCVCT, the distribution of HBV graiotype was
significantly different between ciiriiotic-HCC patients and non cirrhotic-HCC patients. Genotype
C (88.9%) was predominantly detected among cirriiotic-HCC patients, wliile genotype B (72.7%)
was mostly idoitified among non cirrhotic-HCC patients (p=0.01).
