KEANEKARAGAMAN GENETIK RAMIN (Gonystylus bancanus Miq. Kurz) DI CAGAR BIOSFER GIAM SIAK KECIL-BUKIT BATU KABUPATEN BENGKALIS PROVINSI RIAU BERDASARKAN POLA PITA ISOZIM

Abstract

Ramin is timber plant with high demand in furniture industries because it has good wood and fiber texture. The high selling prices and market demand has led to increase illegal logging, consequently ramin population will be endangered. Therefore, it is necessary to study the genetic diversity as one of the conservation effort of ramin using biochemical markers. This study was aimed to analyze the genetic diversity of ramin (Gonystylus bancanus Miq. Kurz) in Giam Siak Kecil-Bukit Batu Bengkalis biosphere reserves using an isozymes technique. Isozyme analysis was performed using the horizontal model of starch gel electrophoresis. A total of 50 individual ramins were used for analyzing the isozyme banding patterns using one enzyme namely peroxidase (PER). The result showed clear banding patterns. PER enzyme produced twelve banding patterns and migrated to the anode (positive) and cathode (negative) poles. SAHN clustering analysis using UPGMA method showed that all of the individuals of ramin were clustered with the similarity coefficient of 38% and separated into two groups (I and II). All of the members of first cluster were grouped with the similarity coefficient 61%, while the second cluster members were grouped with similarity coefficient 55%. This data indicated that the genetic diversity of ramin in biosphere reserves Giam Siak Kecil-Bukit Batu Bengkalis Regency of Riau Province was quite high