INDUKSI TUNAS IN VITRO DARI EKSPLAN TUNAS BUAH ( SLIP) TANAMAN NANAS (Ananas comosus (L.) Merr.) ASAL KAMPAR DENGAN PENAMBAHAN 6-BENZYLAMINOPURINE (BAP)

Abstract

Pineapple ( Ananas comosus (L.) Merr.) is widely cultivated. One of pineapple plantation centers in Riau Province is the Kampar district. The vegetative seedling technique of pineapple is done using tissue culture techniques. Pineapple plant part that used as explants in this s tudy is fruit buds. Explants were cut into whole bud and bud sectioned longitudinally . The growth regulator used was BAP with different concentration, i.e.0.0, 0.5, 1.0, 1.5, 2.0, 2.5 mg/l in Murashige and Skoog (MS )medium. This study aims to determine the optimum concentration of BAP as well as the best explant part and to get the best combination between treatments in shoot induction. The study design used randomized block design ( RBD) factorial. The concentration of 1.0 mg/l BAP on whole bud explants induced 3 buds, while t he bud sectioned longitu dinally induced on ly one shoot on control, and concentration at 0.5 and 1.0 mg/l. Whole bud explants induced shoots in all of the treatments except the control. The interaction between treatment and the combination that could induced the greatest number of shoo t was the whole bud explants with the addition of 1.0 mg/l BAP.