ISOLASI DAN UJI TOKSISITAS METABOLIT SEKUNDER EKSTRAK n-HEKSANA KULIT BATANG TUMBUHAN Polyalthia pulchra var. angustifolia King (ANNONACEAE)

Abstract

The secondary metabolites of the stem bark of Polyalthia pulchra were isolated and than their toxicity activity assayed using Brine Shrimp Lethality Test (BSLT) method. The sample was extracted by maceration method using n-hexane solvent. Structures of the isolated compounds were conducted using Gas Chromatography and Mass Spectrometry (GC-MS). The dried sample produced 3.21 g n-hexane extract (0.25%) and 0.032 g 2nd fraction of n-hexane (FH2) (0.0025%). The toxicity assay showed that n-hexane extract and FH2 fraction was toxic with LC50 5.9 and 7.1 ppm. From the results of GC–MS analysis, it is shown that FH2 fraction contains campesterol, stigmasterol, and γ-sitosterol compounds with retention times 75.605, 76.568 and, 78.172 minutes, respectively.