IDENTIFIKASI DAN UJI AKTIVITAS TOKSISITAS EKSTRAK n-HEKSANA DARI KULIT BATANG TUMBUHAN Xylopia malayana Hook. f.et Thomson (ANNONACEAE)

Abstract

The plant family of Annonaceae spreads out widely in Indonesia. This family is best known for its secondary metabolite resource. Xylopia malayana, a species of Annonaceae can be found in Pekanbaru. The objective of this study was to isolate secondary metabolite from the stem bark. It has been carried out using maceration method with n-hexane as the solvent. Separation of the metabolites has been done using vacuum liquid chromatography (VLC) and column chromatography. Nine combined fractions were produced The extract subjected to VLC were obtained nine combined by VLC fraction 1 (Fg 1) to fraction 9 (Fg 9). Fg 2 was analyzed using gas chromatography-mass spectrometry (GC-MS) resulting 35 peaks of coumpounds. This fraction contained some important volatile oils such as caryophyllene oxide (7,40%), 3,4-dimethyl, 3-cyclohexen- -carboxaldehyde (4,98%), valerenal (15,49%), 2-methyl-4- (2,6,6-trimethyl-1-cyclohexen-1-yl)-2-butenal (8,37%), hexadecanoic acid (4,31%) and oleic acid (8,14%). The result from toxicity test by BLST method of nine fractions showed only Fg 1 had high toxicity (LC50 = 50,11 ppm)