SEKUEN GEN FERRITIN PARSIAL PADA VARIETAS PADI DARI PROVINSI RIAU TERKAIT HOMEOSTASIS FE PADA LAHAN RAWA PASANG SURUT

Abstract

Iron is an essential micronutrient for plant growth and productivity. However, excess Fe is toxic, causing multiple damage to celluler structures. Commonly, tidal wetlands rice variety relative tolerance to waterlogged conditions and excess Fe. Mechanism of plants to tolerate the excess Fe is related to the action of ferritin protein. The objective of this study was to compare partial ferritin gene sequence between riau rice variety with national tidal wetlands rice variety, Siam Sintanur. This research was conducted through DNA extraction with CTAB method, amplification using forward primer OsFer_E5 and reverse primer OsFer_E8, sequencing, sequence analysis using BLAST and ClustalW program. There was a SNP (single nucleotide polymorphism) of partial ferritin gene sequence at base-114. IR64 and Siam Sintanur had base-Adenin while Bakung, Siputih, and Serei had base-Citosin at that position. Further investigations need to be done