Abstract:
Giam Siak Kecil-Bukit Batu (GSK-BB) Biosphere Reserve is one of peatland area in
Riau Province. Peat soil contains high organic matter consisting of lignin, pectin,
cellulose and hemicellulose. Lignin, a natural organic polymer, is abundant in nature, but it is difficult to degraded. The way to overcome this problem is by utilizing lignin degrading bacteria. So far, the ability of cellulolytic bacteria isolated from peat soil of GSK-BB Biosphere Reserve in lignin degradation is still unknown. Therefore, it is necessary to analyze the potential of cellulolytic bacteria collection in degrading lignin. Number of isolates analyzed is about 61 isolates. Semiquantitatively, ligninase enzyme
activity by used of spot method employing guaiacol enrichment medium showed that 50
isolates are able to degrade lignin. As many as 12 isolates (24.0%) revealed high
potential in lignin degradation based on median value test. Isolate PPA KBB 10-4B11
has the highest ligninase activity with a clear zone/colony diameter ratio value of 24.0.
