Senyawa Flavonoid Dari Ekstrak Etil Asetat Kulit Batang Tumbuhan Bauhinia hullettii Prain Dan Uji In Vitro Sel Murine Leukemia P-388

Abstract

Isolation, characterization, and in vitro testing against murine leukemia P-388 cell of flavonoid compounds from Bauhinia hullettii Prain stem bark have been performed. Isolation was carried out by extraction and fractionation. Extraction used meceration method at room temperature to obtain n-Heksana extract, dicloromethane extract, and etil acetate extract. Based on phytochemical testing, etil acetate extract contain flavonoid compounds. Extraxct etil acetate was fractionated by vacum column chromatography, gravitation column chromatography, and flash column chromatography. The fractionation resulted yellow crystal that having melting point of 188 o C - 189 o C. The in vitro testing against murine leukimia P-388 cell of isolated flavonoid compounds show “medium” activity with IC 50 = 22,5 μg/ml.