SINTESIS SENYAWA KURKUMIN TURUNAN 1-BENZIL-4-PIPERIDINON DAN UJI TOKSISITAS DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT)

Abstract

Curcumin is one of secondary metabolites classified into the class of fenolic that are known to have biological activities such as anti-oxidant, anti-diabetic, anti-inflammatory and anti-cancer. Therefore, many curcumins become model of target compounds with variation structure. Curcumins of 1-benzyl-piperidin-4-on derivative were synthesized using the microwave irradiation with a base catalyst (NaOH) and an acid catalyst metanasulfonat. The rendement result of curcumins analog were as followed: (3E,5E)-3,5-bis(2’-klorobenzilidin)-1-benzil-4-piperidinone (1) about 93,03%, (3E,5E)-3,5-bis(4’-klorobenzilidin)-1-benzil-4-piperidinone (2) about 14,40% and (3E,5E)-3,5-bis(3’-bromobenzilidin)-1-benzil-4-piperidinone (3) about 33%. The purity of all compounds has been tested using TLC, melting point test and analytical HPLC. Then, they were identified using UV, IR, 1NMR and MS spectroscopy. The toxicity test of curcumins was done by Brine Shrimp Lethality Test (BSLT) method which was indicated by LC50 <200 μg/mL. Between the three analog curcumins only (1) has potensial as anti-cancer by LC50= 22,44 μg/mL.