Abstract:
The research about validation of quantitative analysis method of erythromycin stearate using spectrophotometry UVVis
after added of gentian violet has been carried out. The measurement was done at wavelength 637 nm in methanolborate
buffer (40:60). Result show that the mean of recovery was 100,826% (w/w), the difference of level after several
measurement was 0,637% and difference mean statistically was 0,159%, SD 1,751 and KV 1,736%, the regression
equalization y = -0,0456 + 0,001347; regression (r) 0,999, Sy/x 0,0099, limit of detection was 20,668 μg/ml and limit of
quantitative was 68,894 pg/ml. The quantitative analysis of erythromycin stearate tablet observed that the mean of
recovery was 99,176% (w/w), SD 0,958 and KV 1,736%. The result of the experiment showed that this method was
valid as quantitative analysis method for erythromycin stearate.
