PENGARUH TEMPERATUR LIQUIFIKASI TERHADAP PEMBUATAN BIOETANOL DARI PATI SORGUM DENGAN PROSES SAKARIFIKASI DAN FERMENTASI SERENTAK DENGAN YEAST CANDIDA UTULIS

Abstract

Bioethanol is produced from plant raw materials containing sugar, starch and cellulose, through the fermentation process with the help of microorganisms. One of the raw materials that have the potential to be developed in Indonesia is sorghum seeds that contain 65% -71% starch, so it can be done liquification process using enzymes amylase and the simultaneous saccharification fermentation (SSF) using enzyme glucoamylase and yeast Candida utilis as agents fermentation to produce bioethanol. The purpose of this study to determine the effect of temperature liquification to sugar produced and determine the best fermentation time. This research is varying liquification temperatures, that is 750C, 850C and 950C and the fermentation time is 12, 24, 48 and 72 hours at pH 5. Bioethanol tests were conducted with alcoholmeter and sugar analysis using visible light spectrophotometer. The results showed that the highest concentration of sugar obtained at the liquification temperature of 850C, using the enzyme amylase reached 4.63 g/l, while the best fermentation time was 48 hours at liqufication temperature 850C with bioethanol concentration of 4% or 40 g/l.