Abstract:
Bioethanol is produced from plant raw materials containing sugar, starch and
cellulose, through the fermentation process with the help of microorganisms. One
of the raw materials that have the potential to be developed in Indonesia is
sorghum seeds that contain 65% -71% starch, so it can be done liquification
process using enzymes amylase and the simultaneous saccharification
fermentation (SSF) using enzyme glucoamylase and yeast Candida utilis as agents
fermentation to produce bioethanol. The purpose of this study to determine the
effect of temperature liquification to sugar produced and determine the best
fermentation time. This research is varying liquification temperatures, that is
750C, 850C and 950C and the fermentation time is 12, 24, 48 and 72 hours at pH
5. Bioethanol tests were conducted with alcoholmeter and sugar analysis using
visible light spectrophotometer. The results showed that the highest concentration
of sugar obtained at the liquification temperature of 850C, using the enzyme
amylase reached 4.63 g/l, while the best fermentation time was 48 hours at
liqufication temperature 850C with bioethanol concentration of 4% or 40 g/l.
