Variasi Konsentrasi Substrat Pati Sorgum Menjadi Bioetanol dengan Proses Sakarifikasi dan Fermentasi Serentak Menggunakan Enzim StargenTM 002 Dan Yeast Saccharomyces Cerevisiae

Abstract

Sorghum is one of the agricultural commodities used for biofuels. Sorghum is a resource of grain yield 55-75% starch composition similar to corn, which is potetianlly as raw material for production of bioethanol. Research based sorghum bioethanol manufacture carried out using enzymes stargenTM 002 and starch raw materials. The purpose of this study was to determine the concentration of substrate and the best time of fermentation to produce bioethanol. The research method consists of hydrolysis and saccharification processes are aided by enzymes stargenTM 002 followed by a fermentation process using yeast Saccaromyces Cerevisiaee , ferment at room temperature, pH 4.5, enzymes stargenTM 002 concentration 2,% and distilled at a tempaerature 78 -800C. variable substrate concentration used were 30 g/l; 40 g/l; 50 g/l; and 60 g/l and time of fermentation were 12; 24; 48 and 72 hours. The result indicate that the substrate concentration 0f 30 g/l and the optimum time is 48 hours can produce the higest bioethanol concentration of 6% (v/v).