Optimalisasi Komposisi Media dan Konsentrasi Sumber Karbon Produksi Enzim Selulase Bakteri Selulolitik Strain Lokal S-16 dan S-22

Abstract

Isolates of S-16 and S-22 are some of the local strains producing cellulases that have been isolated from the Siak River water. Cellulase enzyme can be degrades of cellulose waste containing that enable for the processing of waste to economically priced products such as glucose. In this research, optimization of the composition production of cellulase enzymes from isolates of S-16 and S-22 with a variety of media production and concentration of carbon source production. The quantitative assay for enzyme activity was determined by Nelson Somogyi method with carboxymethyl cellulose (CMC) as a substrate. The highest cellulase enzyme activity of S-16 obtained in a media production (KH2PO4; MgSO4.7H2O; NaNO3; CaCl2; NaCl; CMC) of 88x10-3 U/mL, and the optimum concentration of source of carbon at 1% obtained for 42x10-3 U/mL, whereas of S-22 obtained in a media production (KH2PO4; MgSO4.7H2O; FeSO4.7H2O; NaNO3; KCl; CMC) of 14x10-3 U/mL, and the optimum carboxymethil Cellulosa concentration of sources of carbon at 1% obtained for 16x10-3 U/mL. Comparison of cellulase enzyme activity of isolate S-16 and S-22 by using Duncan test multiple range at level 5%