Abstract:
Nosocomial Infection or Health Care Associated Infection (HCAI) is one of the leading
causes of mortality and morbidity in hospitals and the other health care facilities. Washing
hands is the way to reduces incidence of HCAI. World Health Organization (WHO) recommends
the use of alcohol-based handrub because its antibacterial activity had been tested in clinical
and WHO has identified formulations for the local preparation. This is an laboratory
experimental study aimed to determine the antibacterial activity of directly used World Health
Organization (WHO)-recommended handrub formulation ( WHO I ) and used 40 days after
production ( WHO II ) to 20 subjects. Each subject wash their hand using directly used World
Health Organization (WHO)-recommended handrub formulation and used 40 days after
production and then do the test of antibacterial avtivity and count the bacterial colonies by Total
Plate Count method. The results of this study showed that significantly log 10 decrease of
bacterial colonies in pre and post-handrubbing using directly used World Health Organization
(WHO)-recommended handrub formulation and used 40 days after production. Not-significantly
difference of log 10 decrease using directly used World Health Organization (WHO)-
recommended handrub formulation and used 40 days after production.
