PENGARUH TEMPERATUR LIKUIFIKASI KONVERSI PATI SORGUM MENJADI GULA dan WAKTU SIMULTANEOUS SACCHARIFICATION and FERMENTATION (SSF) PRODUKSI BIOETANOL

Abstract

Sorghum is one of the potential materials in the manufacture of bioethanol. Sorghum has advantages over sugarcane and maize, that is a shorter harvest time, needs little water and a source of ethanol can be obtained from the sap, starch and pulp. The purpose of this study to determine the effect of temperature liquefications of sugar produced and determine the best fermentation time. Part of sorghum is used as raw material for bio-ethanol is grain sorghum containing 73% carbohydrate by immersion NaOH to remove proteins in grain sorghum that can inhibit the action of enzymes. The process used is liquefications and simultaneous saccharification and fermentation by varying the temperature of liquefications, that is 75 °C, 85 °C and 95 °C and Simultaneous Saccharification and Fermentation time is 12, 24, 48 and 72 hours. Fermentation was carried out with the help of yeast Saccharomyces cerevisiae. Tests were conducted with alkoholmeter and sugar analysis using visible spectrophotometer results showed that the highest sugar yield is 4.685 g/l at a temperature of liquefications 95 °C and the highest ethanol concentration of 70 g/l at 72 hours with the temperature fermentation liquifikasi 95 °C