ISOLASI METABOLIT SEKUNDER DAN UJI PENGHAMBATAN ENZIM α-GLUKOSIDASE DARI EKSTRAK n-HEKSANA Plectranthus ciliatus

Abstract

Plectranthus ciliatus is one of the species belonging to the family Lamiaceae. Two Plectranthus species, P. amboinicus and P. ecklonii have been reported to possess inhibitory activity of the enzyme α-glucosidase. This study aimed to isolate secondary metabolites and test α-glucosidase inhibition from n-hexane extract of P. ciliatus. This species was extracted by maceration using methanol and partitioned with n-hexane, followed by fractionation, purification and characterization. These extracts were dried and tested in vitro with inhibition α-glucosidase. The results of the study obtained a pure compound of white needle crystals with a melting point of 144-146°C (coded PC-H-V5-FL17). The results of the characterization with the FTIR spectra and phytochemical analysis of the PC-H-V5-FL17 were assumed to be a β-sitosterol. The results with inhibition percentage values 31% for n-hexane extract, -13% for PC-H-V5- FL17, and 97.8% for acarbose. Based from the data the extract and isolated compound showing weak and inactive inhibition compared to positive control of acarbose.