Abstract:
Plectranthus ciliatus is one of the species belonging to the family Lamiaceae. Two
Plectranthus species, P. amboinicus and P. ecklonii have been reported to possess
inhibitory activity of the enzyme α-glucosidase. This study aimed to isolate secondary
metabolites and test α-glucosidase inhibition from n-hexane extract of P. ciliatus. This
species was extracted by maceration using methanol and partitioned with n-hexane,
followed by fractionation, purification and characterization. These extracts were dried
and tested in vitro with inhibition α-glucosidase. The results of the study obtained a
pure compound of white needle crystals with a melting point of 144-146°C (coded
PC-H-V5-FL17). The results of the characterization with the FTIR spectra and
phytochemical analysis of the PC-H-V5-FL17 were assumed to be a β-sitosterol. The
results with inhibition percentage values 31% for n-hexane extract, -13% for PC-H-V5-
FL17, and 97.8% for acarbose. Based from the data the extract and isolated compound
showing weak and inactive inhibition compared to positive control of acarbose.
